01; Syngene, Frederick, MD, USA) after 5 s exposure for housekeeping genes and 25 min exposure for TSP-1, nucleolin and endostatin. Protein expression was quantified using NIH ImageJ Software (version 1.62) and expressed as densitometric arbitrary units (A.U.). The same control samples were always loaded on every gel in order to allow normalization of densitometry across blots and allow all groups