six ml/min in 0.5 trifluoroacetic acid in HPLC grade water onto a nano trap column (100 mm i.d. 62 cm, packed with Acclaim PepMap RSLC C18, three mm, 100 A, Thermo Fisher Scientific). Immediately after five minutes, peptides had been eluted and separated on the analytical columnMechanisms Restricting ARL-13 to Ciliary Membranes(Acclaim PepMap RSLC C18, two mm, one hundred A, 75 mm i.d. 625 cm, na