Sp1GFP cells expressed low levels of epithelial (E) cadherin, which can be consis tent with the notion that Mesp1GFP cells undergo EMT throughout MCP specification (Fig. 5 C). RTPCR evaluation performed on FACSisolated CXCR4/PDGFRa/Flk1 TP cells showed that MCPs isolated working with monoclonal antibodies present a related enrichment for the expression of cardiovascular transcriptional regulators